Yamaguchi A, Urano T, Goi T, Takeuchi K, Niimoto S, Nakagawara G, Furukawa K, Shiku H
First Department of Surgery, Fukui Medical School, Japan.
Cancer. 1994 May 1;73(9):2280-4. doi: 10.1002/1097-0142(19940501)73:9<2280::aid-cncr2820730908>3.0.co;2-3.
The expression of nm23-H1 and nm23-H2 proteins in 25 hepatocellular carcinomas was studied immunohistochemically.
Tissue specimens were reacted with anti-human nm23-H1 and nm23-H2 monoclonal antibodies (MoAb) (H1-229 and H2-206, respectively) and then stained by the biotin-streptoavidin complex method.
Adjacent nontumorous tissues were intensely stained with nm23-H1 and nm23-H2. Of the 25 hepatocellular carcinomas, 60% were positive for MoAb H1-229, and 68% were positive for MoAb H2-206. These immunoreactivities were most common in the cytoplasm of tumor cells. There was no significant correlation between the expression of nm23-H1 protein and tumor size, Edmondson's histopathologic classification, or invasion of the capsule. However, the authors observed an inverse relationship between nm23-H1 expression and intrahepatic metastases of hepatocellular carcinomas. There was no significant correlation between the expression of nm23-H2 protein and clinicopathologic findings. Only a short survival period was observed in patients with hepatocellular carcinoma with reduced nm23-H1 or nm23-H2 proteins.
The results suggest that nm23-H1 protein plays a role in the suppression of intrahepatic metastasis of hepatocellular carcinoma and that the combined expression of nm23-H1 is associated with favorable prognosis.
采用免疫组织化学方法研究了25例肝细胞癌中nm23-H1和nm23-H2蛋白的表达情况。
组织标本分别与抗人nm23-H1和nm23-H2单克隆抗体（MoAb）（分别为H1-229和H2-206）反应，然后用生物素-链霉亲和素复合物法染色。
nm23-H1和nm23-H2在癌旁非肿瘤组织中呈强阳性染色。在25例肝细胞癌中，60%对MoAb H1-229呈阳性，68%对MoAb H2-206呈阳性。这些免疫反应在肿瘤细胞的细胞质中最为常见。nm23-H1蛋白的表达与肿瘤大小、Edmondson组织病理学分级或包膜侵犯之间无显著相关性。然而，作者观察到nm23-H1表达与肝细胞癌肝内转移之间呈负相关。nm23-H2蛋白的表达与临床病理结果之间无显著相关性。nm23-H1或nm23-H2蛋白表达降低的肝细胞癌患者生存期较短。
结果表明nm23-H1蛋白在抑制肝细胞癌肝内转移中起作用，且nm23-H1的联合表达与良好预后相关。
