Emmings F G, Evans R T, Genco R J
Infect Immun. 1975 Aug;12(2):281-92. doi: 10.1128/iai.12.2.281-292.1975.
The antibody response of Macaca fascicularis in parotid saliva and serum to local immunization by two routes with Streptococcus mutans was studied and compared over 1 year. Antibodies were titrated and classified by indirect immunofluorescent staining using specific antiglobulin conjugates. Antiglucosyltransferase activity was assayed by an enzyme inhibition test. Animals were immunized first by injecting formalin-killed bacterial cells and cell products subcutaneously into the vicinity of the four major salivary glands. The monkeys were next immunized by retrograde instillation of antigen into the parotid duct. Extensive subcutaneous local immunization gave a serum response only. After parotid duct immunization, high titers of immunoglobulin A (IgA) antibody, along with traces of immunoglobulin G (IgG) immunoglobulin M (IgM) antibody, appeared in the parotid saliva, and in the serum high titers of IgG antibody were present along with lower titers of IgA and IgM. IgA antibodies in parotid fluid were shown by double immunofluorescent staining to be associated with antigenic determinants which cross-reacted with an antiserum directed to human secretory component. Titers in parotid fluids and sera fell sharply when immunization was stopped. This response pattern was reproducible. High concentrations of antibody capable of inhibiting glucosyltransferase prepared from S. mutans were found in the sera, but relatively little was detected in the parotid fluids. Extensive immunization via the parotid duct resulted in transient functional impairment of the gland, as evidenced by diminished salivary flow rates. We conclude that parotid ductal immunization can be an effective method for stimulating a salivary secretory IgA antibacterial antibody response.
对食蟹猴腮腺唾液和血清中针对变形链球菌经两种途径进行局部免疫的抗体反应进行了为期1年的研究和比较。使用特异性抗球蛋白结合物通过间接免疫荧光染色对抗体进行滴定和分类。通过酶抑制试验测定抗葡糖基转移酶活性。首先通过将福尔马林灭活的细菌细胞和细胞产物皮下注射到四大唾液腺附近对动物进行免疫。接下来通过将抗原逆行滴注到腮腺导管中对猴子进行免疫。广泛的皮下局部免疫仅产生血清反应。腮腺导管免疫后，腮腺唾液中出现高滴度的免疫球蛋白A（IgA）抗体以及微量的免疫球蛋白G（IgG）和免疫球蛋白M（IgM）抗体，血清中则存在高滴度的IgG抗体以及较低滴度的IgA和IgM。通过双重免疫荧光染色显示，腮腺液中的IgA抗体与抗原决定簇相关，这些抗原决定簇与针对人分泌成分的抗血清发生交叉反应。停止免疫后，腮腺液和血清中的滴度急剧下降。这种反应模式是可重复的。在血清中发现了高浓度的能够抑制从变形链球菌制备的葡糖基转移酶的抗体，但在腮腺液中检测到的相对较少。通过腮腺导管进行广泛免疫导致腺体出现短暂的功能损害，唾液流速降低证明了这一点。我们得出结论，腮腺导管免疫可以是刺激唾液分泌性IgA抗菌抗体反应的有效方法。
