Santini C, Tidu V, Tognon G, Ghiretti Magaldi A, Bassi R
Dipartimento di Biologia, Università di Padova, Italy.
Eur J Biochem. 1994 Apr 1;221(1):307-15. doi: 10.1111/j.1432-1033.1994.tb18742.x.
The three-dimensional structure of photosystem II (PSII) has been determined by conventional transmission electron microscopy and computerized three-dimensional reconstruction. Both the complete system and that lacking the oxygen-evolving complex have been analyzed. The PSII complex has a four-lobed structure with twofold symmetry. An estimate of the molecular mass and the results of Deriphat/PAGE analysis suggest that a reaction centre is present in each half of the structure resolved by electron microscopy. Stepwise removal of components of the complex showed that the removal of CP47 (a 47-kDa chlorophyll-protein complex) induces monomerization of PSII, which indicates the importance of this subunit for the dimeric structure.
通过传统透射电子显微镜和计算机三维重建技术确定了光系统II（PSII）的三维结构。对完整系统以及缺少放氧复合体的系统均进行了分析。PSII复合体具有四重对称的四叶结构。分子量估算结果和十二烷基肌氨酸钠/聚丙烯酰胺凝胶电泳（Deriphat/PAGE）分析结果表明，在通过电子显微镜解析的结构的每一半中都存在一个反应中心。逐步去除复合体的各个组分表明，去除CP47（一种47 kDa的叶绿素-蛋白复合体）会导致PSII单体化，这表明该亚基对二聚体结构具有重要意义。
