Kromer-Metzger E, Lagueux M
UPR CNRS Réponse immunitaire et Développement chez les insectes, Institut de Biologie Moléculaire et Cellulaire, Strasbourg, France.
Eur J Biochem. 1994 Apr 1;221(1):427-34. doi: 10.1111/j.1432-1033.1994.tb18755.x.
A peptide with significant sequence similarity to mammalian insulins, Locusta insulin-related peptide (LIRP) has recently been isolated from neurohaemal lobes of Locusta migratoria and the corresponding cDNA has been cloned. We report here the cloning of the LIRP gene and we show that this gene is present as a single copy/haploid genome. The organization of this gene is similar to that of mammalian insulin genes. There are at least two LIRP transcripts, LIRP T1 and LIRP T2, differing in their 5' untranslated regions and in the 5' end of the coding region resulting in an additional ATG present in LIRP T2. The two transcripts are differentially expressed in Locusta; whereas LIRP T1 is only expressed in neurosecretory cells of the brain, LIRP T2 is present at low levels in all tissues. Our data indicate that the LIRP gene has at least two promoters, the alternative usage of which could account for a differential regulation of expression of LIRP in the neurosecretory cells and in various peripheral tissues.
一种与哺乳动物胰岛素具有显著序列相似性的肽，即飞蝗胰岛素相关肽（LIRP），最近已从飞蝗的神经血叶中分离出来，并且相应的cDNA已被克隆。我们在此报告LIRP基因的克隆，并表明该基因以单拷贝/单倍体基因组形式存在。该基因的结构与哺乳动物胰岛素基因的结构相似。至少有两种LIRP转录本，即LIRP T1和LIRP T2，它们在5'非翻译区和编码区的5'端存在差异，导致LIRP T2中存在一个额外的ATG。这两种转录本在飞蝗中差异表达；LIRP T1仅在大脑的神经分泌细胞中表达，而LIRP T2在所有组织中的表达水平较低。我们的数据表明，LIRP基因至少有两个启动子，其交替使用可能解释了LIRP在神经分泌细胞和各种外周组织中表达的差异调节。
