Lagueux M, Lwoff L, Meister M, Goltzené F, Hoffmann J A
Unité Associée au Centre National de la Recherche Scientifique 672, Université Louis Pasteur, Strasbourg, France.
Eur J Biochem. 1990 Jan 12;187(1):249-54. doi: 10.1111/j.1432-1033.1990.tb15302.x.
From neurohaemal lobes of corpora cardiaca of Locusta migratoria a 5-kDa peptide has been isolated and its sequence established [see the accompanying paper, by Hietter et al. (1990) Eur. J. Biochem. 187, 241-247]. We have designed oligonucleotide probes from the peptide sequence of this molecule and screened a library prepared from mRNA of the neurosecretory cell region of the brain of this insect. Several positive cDNAs were isolated, the combined nucleotide sequences of which predict a large precursor of 145 residues (15770 Da) containing the newly isolated 5-kDa peptide. The peptide is flanked by regions homologous to the A and B chains of the superfamily of insulins. The overall organization of the precursor is as follows: signal peptide/domain homologous to the B chain of insulins/C (connecting)-peptide (corresponding to the newly isolated 5-kDa peptide)/domain homologous to the A chain of insulins. The numbers and relative positions of the cysteines of the Locusta peptide are equivalent to those of the other members of the insulin superfamily and most of the hydrophobic core residues are conserved.
从飞蝗心侧体的神经血叶中分离出一种5千道尔顿的肽，并确定了其序列[见Hietter等人（1990年）的附文，《欧洲生物化学杂志》187卷，241 - 247页]。我们根据该分子的肽序列设计了寡核苷酸探针，并筛选了一个由这种昆虫大脑神经分泌细胞区域的mRNA制备的文库。分离出了几个阳性cDNA，其组合核苷酸序列预测有一个由145个残基组成的大前体（15770道尔顿），其中包含新分离的5千道尔顿的肽。该肽两侧是与胰岛素超家族A链和B链同源的区域。前体的总体结构如下：信号肽/与胰岛素B链同源的结构域/C（连接）肽（对应于新分离的5千道尔顿的肽）/与胰岛素A链同源的结构域。飞蝗肽中半胱氨酸的数量和相对位置与胰岛素超家族的其他成员相同，并且大多数疏水核心残基是保守的。
