耳蜗中的G蛋白Gi2α：克隆及其在受体细胞中的选择性表达。

G protein Gi2 alpha in the cochlea: cloning and selective occurrence in receptor cells.

作者信息

Tachibana M, Asano T, Wilcox E, Yokotani N, Rivolta M N, Fex J

机构信息

Laboratory of Molecular Biology, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892.

出版信息

Brain Res Mol Brain Res. 1994 Feb;21(3-4):355-8. doi: 10.1016/0169-328x(94)90267-4.

DOI:10.1016/0169-328x(94)90267-4

PMID:8170357

Abstract

A cDNA library was made from the mouse cochlea and screened with a G protein-cDNA like molecule obtained from cochlear tissue by polymerase chain reaction. The nucleotide sequence of a clone, named cochlear Gi2 alpha, had 99.2% identity to mouse macrophage Gi2 alpha. Using an antibody which is selective for Gi2 alpha, expression of the cochlear Gi2 alpha was localized in outer and inner hair cells of the organ of Corti. Possible functional roles of this G protein in hair cells are discussed.

摘要

从小鼠耳蜗制备了一个cDNA文库，并用通过聚合酶链反应从耳蜗组织获得的一种类似G蛋白-cDNA的分子进行筛选。一个名为耳蜗Gi2α的克隆的核苷酸序列与小鼠巨噬细胞Gi2α有99.2%的同一性。使用对Gi2α具有选择性的抗体，耳蜗Gi2α的表达定位于柯蒂氏器的外毛细胞和内毛细胞。讨论了这种G蛋白在毛细胞中的可能功能作用。