Sideropoulos A S, Specht S
Department of Biological Sciences, Duquesne University, Pittsburgh, Pennsylvania 15282.
Microbios. 1994;77(312):167-79.
Acetone extracts of crude and size-classified airborne particulates were tested for mutagenicity using the Ames Salmonella typhimurium microsomal bioassay. Both direct and indirect acting frameshift and base-substitution mutagens were detected. Size fractionation unveiled direct and indirect acting mutagens which were concealed in the crude preparation, and demonstrated that the mutagenic potential of the crude extract increased as the particle size decreased (0.95-0.49 micron). The mutagenicity observed in this fraction increased by approximately 1.8-fold and 2.0-fold of that found in the crude extract of the same sampling site with S-9 activation, thus warranting further study of the mutagenic interactions present in complex environmental airborne pollutants. Approximately 41.4% of the deposited particulate mass was extractable by acetone. Furthermore, 67% of this extractable mass was derived from particles of < 1.5 microns diameter which contained the bulk (73%) of mutagenic activity.
使用鼠伤寒沙门氏菌微粒体生物测定法对粗制的和按大小分级的空气传播颗粒的丙酮提取物进行致突变性测试。检测到了直接和间接作用的移码突变剂和碱基取代突变剂。粒度分级揭示了隐藏在粗制制剂中的直接和间接作用的突变剂，并表明粗提取物的致突变潜力随着粒径减小（0.95 - 0.49微米）而增加。在该级分中观察到的致突变性在有S - 9激活的情况下比同一采样点粗提取物中的致突变性分别增加了约1.8倍和2.0倍，因此有必要进一步研究复杂环境空气传播污染物中存在的致突变相互作用。大约41.4%的沉积颗粒物质可被丙酮提取。此外，这种可提取物质的67%来自直径小于1.5微米的颗粒，这些颗粒含有大部分（73%）的致突变活性。
