Torre D, Pugliese A, Ferrario G, Marietti G, Forno B, Zeroli C
Division of Infectious Diseases, Regional Hospital, Varese, Italy.
FEMS Immunol Med Microbiol. 1994 Feb;8(2):127-31. doi: 10.1111/j.1574-695X.1994.tb00434.x.
Fibronectin (FN) is present in soluble and matrix forms in various body fluids and tissues, and has been shown to bind to several pathogens, including viruses. The interaction of FN with viral proteins of human immunodeficiency virus (HIV-1) was investigated by immunofluorescence technique using a cell line chronically infected with HIV-1 (H9-V). The results of this study showed that FN binds to HIV-1 infected cells, especially at FN concentration of 5 micrograms/ml. In addition, FN-pentapeptide has shown the ability to bind to HIV-1 infected cells. On the other hand, preincubation with antibodies against FN abolished the binding of FN to HIV-1 infected cells. Finally, FN has shown to bind to HIV-1 glycoproteins, including gp41 and gp120. In contrast, no binding to HIV-1 core proteins, including p15 and p24, was noted. We suggest that FN, in binding HIV-1 particles, may reduce viremia and thus may be involved in the clearance of viral proteins from the cells.
纤连蛋白（FN）以可溶形式和基质形式存在于各种体液和组织中，并且已证明它能与包括病毒在内的多种病原体结合。利用长期感染人类免疫缺陷病毒1型（HIV-1）的细胞系（H9-V），通过免疫荧光技术研究了FN与HIV-1病毒蛋白的相互作用。这项研究的结果表明，FN能与HIV-1感染的细胞结合，尤其是在FN浓度为5微克/毫升时。此外，FN五肽已显示出与HIV-1感染细胞结合的能力。另一方面，用抗FN抗体进行预孵育消除了FN与HIV-1感染细胞的结合。最后，FN已证明能与HIV-1糖蛋白结合，包括gp41和gp120。相比之下，未观察到与HIV-1核心蛋白（包括p15和p24）的结合。我们认为，FN在结合HIV-1颗粒时，可能会降低病毒血症，因此可能参与从细胞中清除病毒蛋白。
