Rifai N, Fuller D, Law T, Mikati M
Department of Laboratory Medicine, Children's Hospital, Boston, MA 02115.
Clin Chem. 1994 May;40(5):745-8.
Felbamate, a newly developed antiepileptic agent, has been demonstrated to control partial and generalized seizures effectively. We have developed a gas-chromatographic method for the determination of felbamate, using a wide-bore capillary column, a flame ionization detector, and a simple extraction procedure. The assay day-to-day precision (n = 20) was 5.2% and 3.6% for drug concentrations of 50 and 150 mg/L, respectively; average recovery over a wide range of felbamate concentrations was 95%; the detection limit was 5 mg/L; and assay linearity extended to 300 mg/L. Although 9 of the 27 drugs tested were coextracted with felbamate, they exhibited significantly different retention times and showed no interference. A short-term stability study showed that plasma felbamate is stable at 4, -20, or -78 degrees C for at least 1 month. Plasma felbamate concentrations in 66 pediatric patients ranged from 7 to 154 mg/L (mean +/- SD 44 +/- 24.7). We consider the method ideally suited for therapeutic monitoring of plasma felbamate concentration.
非氨酯是一种新开发的抗癫痫药物，已被证明能有效控制部分性和全身性癫痫发作。我们开发了一种气相色谱法来测定非氨酯，使用宽口径毛细管柱、火焰离子化检测器和简单的萃取程序。对于药物浓度为50和150 mg/L的情况，测定的日常精密度（n = 20）分别为5.2%和3.6%；在很宽的非氨酯浓度范围内平均回收率为95%；检测限为5 mg/L；测定线性范围扩展至300 mg/L。虽然在测试的27种药物中有9种与非氨酯共萃取，但它们的保留时间显著不同，且无干扰。一项短期稳定性研究表明，血浆中的非氨酯在4℃、-20℃或-78℃下至少1个月内稳定。66例儿科患者的血浆非氨酯浓度范围为7至154 mg/L（均值±标准差 44±24.7）。我们认为该方法非常适合用于血浆非氨酯浓度的治疗监测。
