Shihabi Z K, Oles K S
Department of Pathology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157.
Clin Chem. 1994 Oct;40(10):1904-8.
We have developed two methods for determining serum concentrations of felbamate, a new anticonvulsant drug. The first method is based on protein precipitation with acetonitrile, followed by HPLC. The between-run CV for this method is 5.7% (mean 55 mg/L), and the linearity extends from 5 to 175 mg/L. Results by this method compared well with those by an HPLC method based on chloroform extraction (r = 0.98, n = 21). In the second method, based on micellar electrokinetic capillary chromatography, the drug is measured by capillary electrophoresis with direct injection of serum. This method can be completed in 5 min compared with 12 min for the HPLC method, and there is no need for sample extraction. The between-run CV is 5.2% (mean 58 mg/L) and the linearity range is 5-160 mg/L. Results of this direct method correlated well (r = 0.98, n = 37) with those by the HPLC assay. The mean trough serum concentration of felbamate in 123 patients taking this drug was 44.9 mg/L (range 12-129 mg/L).
我们开发了两种测定新型抗惊厥药物非氨酯血清浓度的方法。第一种方法基于用乙腈进行蛋白沉淀，随后进行高效液相色谱分析。该方法的批间变异系数为5.7%（均值55 mg/L），线性范围为5至175 mg/L。该方法的结果与基于氯仿萃取的高效液相色谱法的结果相比吻合良好（r = 0.98，n = 21）。在第二种基于胶束电动毛细管色谱的方法中，通过直接进样血清进行毛细管电泳来测定药物。该方法5分钟即可完成，而高效液相色谱法需要12分钟，且无需样品萃取。批间变异系数为5.2%（均值58 mg/L），线性范围为5 - 160 mg/L。这种直接法的结果与高效液相色谱测定法的结果相关性良好（r = 0.98，n = 37）。123例服用该药物患者的非氨酯血清谷浓度均值为44.9 mg/L（范围12 - 129 mg/L）。
