Clegg J S, Jackson S A, Warner A H
Bodega Marine Laboratory, University of California, Davis, Bodega Bay 94923.
Exp Cell Res. 1994 May;212(1):77-83. doi: 10.1006/excr.1994.1120.
Cells of encysted gastrula embryos of the crustacean Artemia franciscana exhibit extraordinary stability during prolonged anoxia. We find that they contain an abundant protein (referred to as "26-kDa protein") that undergoes translocation to the nucleus during anoxia. The reverse translocation rapidly occurs when anoxic embryos are returned to aerobic conditions. A similar translocation appears to take place in embryos exposed to 42 degrees C aerobic heat shock and prolonged exposure to low temperature (0-2 degrees C), and in diapause embryos. Gel filtration and Western immunoblotting indicate that the 26-kDa protein is translocated to other cellular compartments and may also be associated with a wide variety of "soluble" proteins during anoxia. This protein makes up roughly 15% of the total nonyolk embryo protein and is, by far, most abundant in the encysted embryo stage of the life cycle. The hypothesis is advanced that the 26-kDa protein may play the role of a metabolic regulator and/or a protective molecular chaperone during prolonged anoxia and other forms of stress.
卤虫（Artemia franciscana）包囊化原肠胚的细胞在长时间缺氧期间表现出非凡的稳定性。我们发现它们含有一种丰富的蛋白质（称为“26 kDa蛋白质”），在缺氧期间会转移到细胞核中。当缺氧胚胎恢复到有氧条件时，会迅速发生反向转移。类似的转移似乎也发生在暴露于42摄氏度有氧热休克、长时间暴露于低温（0-2摄氏度）的胚胎以及滞育胚胎中。凝胶过滤和蛋白质免疫印迹表明，26 kDa蛋白质在缺氧期间会转移到其他细胞区室，并且可能还与多种“可溶性”蛋白质相关联。这种蛋白质约占非卵黄胚胎蛋白质总量的15%，并且在生命周期的包囊化胚胎阶段含量最为丰富。有人提出假说，26 kDa蛋白质可能在长时间缺氧和其他形式的应激期间发挥代谢调节剂和/或保护性分子伴侣的作用。
