Kaouass M, Deloyer P, Dandrifosse G
Laboratory of Biochemistry and General Physiology, University of Liège, Belgium.
Digestion. 1994;55(3):160-7. doi: 10.1159/000201142.
The present investigation addresses the question of whether spermine orally given to unweaned rats directly or indirectly exerts its effects on the intestinal brush border disaccharidases and if the adrenal gland secretions play a role in this phenomenon. The results showed that spermine, surgically placed in the lower part of the distal small intestine, induced sucrase, stimulated maltase-specific activity and decreased lactase-specific activity in both proximal and distal segments of the small intestine. Introduction of spermine into the lumen of the large intestine stimulated the specific activities of disaccharidases in the whole small intestine. Intraperitoneal injection had no effect except a slight reduction of lactase-specific activity in the distal intestine. Adrenalectomy prevented the oral effect of spermine on sucrase- and maltase-specific activity but not on lactase-specific activity. Addition of spermine to intestinal explants in organ cultures fails to reproduce any of these effects. It even reduced maltase-specific activity. These findings suggest that dietary polyamines have either direct and indirect effects on properties of rat immature intestine.
本研究探讨了口服给予未断奶大鼠精胺是直接还是间接对肠刷状缘双糖酶产生影响，以及肾上腺分泌是否在此现象中起作用。结果表明，手术置于远端小肠下部的精胺可诱导蔗糖酶、刺激麦芽糖酶的比活性，并降低小肠近端和远端段乳糖酶的比活性。将精胺引入大肠腔可刺激整个小肠双糖酶的比活性。腹腔注射除使远端肠段乳糖酶比活性略有降低外无其他作用。肾上腺切除术可阻止精胺对蔗糖酶和麦芽糖酶比活性的口服效应，但对乳糖酶比活性无影响。在器官培养中向肠外植体添加精胺未能重现上述任何效应，甚至还降低了麦芽糖酶的比活性。这些发现表明，膳食多胺对大鼠未成熟肠道特性具有直接和间接影响。
