Ogino Y, Okada S, Ota Z
Third Department of Internal Medicine, Okayama University Medical School, Japan.
Diabetologia. 1994 Mar;37(3):225-31. doi: 10.1007/BF00398047.
To study the effects of chronic osmotic diuresis which were not associated with hyperglycaemia on the rat kidney, osmotic diuresis was induced by i.v. infusion of urea. A 5 mol/l urea solution was continuously infused at a rate of 100 ml.kg-1 x day-1 on the basis of body weight on day 0. Duration of infusion was 2, 6, 10 or 14 days. Control rats received continuously infused Ringer's solution. Urea-treated groups developed osmotic diuresis (urine flow = about 0.04 ml.min-1 x 100 g body weight-1) comparable to that in rats with experimental diabetes mellitus induced by i.v. streptozotocin (55 mg/kg), however urea-induced osmotic diuresis was not associated with blood glucose level increases. Compared with their controls, rats receiving urea for 2-14 days had markedly increased kidney weight. Rats receiving urea for 10 days showed greatest kidney weight increase, 0.565 +/- 0.044 g/100 g body weight (mean +/- SD), representing a 53% increase compared with the control (0.369 +/- 0.034 g/100 g body weight). Kidney weight was associated with increases in kidney protein content. In contrast, none of control kidney weight values differed significantly from day 0 values (= normal rats; 0.387 +/- 0.028 g/100 g body weight). Creatinine clearance values in urea-treated groups were also higher than those in controls. The maximum value, 0.65 +/- 0.17.ml-min-1 x 100 g body weight-1, was recorded in the 14-day group and was significantly higher than the corresponding control value (0.34 +/- 0.07 ml.min-1 x 100 g body weight-1) (p < 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)
为研究与高血糖无关的慢性渗透性利尿对大鼠肾脏的影响，通过静脉输注尿素诱导渗透性利尿。于第0天，根据体重以100 ml·kg⁻¹·天⁻¹的速率持续输注5 mol/l尿素溶液。输注持续时间为2、6、10或14天。对照大鼠持续输注林格氏液。尿素处理组产生的渗透性利尿（尿流率 = 约0.04 ml·min⁻¹·100 g体重⁻¹）与静脉注射链脲佐菌素（55 mg/kg）诱导的实验性糖尿病大鼠相当，然而尿素诱导的渗透性利尿与血糖水平升高无关。与对照组相比，接受尿素处理2 - 14天的大鼠肾脏重量显著增加。接受尿素处理10天的大鼠肾脏重量增加最大，为0.565±0.044 g/100 g体重（平均值±标准差），相较于对照组（0.369±0.034 g/100 g体重）增加了53%。肾脏重量与肾脏蛋白质含量增加相关。相比之下，对照大鼠的肾脏重量值与第0天的值（=正常大鼠；0.387±0.028 g/100 g体重）相比均无显著差异。尿素处理组的肌酐清除率值也高于对照组。14天组记录到的最大值为0.65±0.17 ml·min⁻¹·100 g体重⁻¹，显著高于相应的对照值（0.34±0.07 ml·min⁻¹·100 g体重⁻¹）（p < 0.001）。（摘要截短于250字）
