Reinisch K M, Chen L, Verdine G L, Lipscomb W N
Gibbs Chemical Laboratory, Harvard University, Cambridge, MA 02138.
J Mol Biol. 1994 May 13;238(4):626-9. doi: 10.1006/jmbi.1994.1319.
A DNA (cytosine)-5-methyltransferase from Haemophilus aegyptius (M.Hae III), which catalyzes methyl transfer from S-adenosyl-L-methionine to DNA, has been crystallized as a covalent complex with a suicide oligonucleotide substrate. Crystals of the co-complex were grown by vapor diffusion with hanging droplets, using polyethylene glycol 3500 as the precipitant. The crystals belong to the orthorhombic space group P2(1)2(1)2(1); the unit cell parameters are a = 57.6 A, b = 108.0 A, c = 155.8 A with two protein-DNA complexes in the asymmetric unit. Complete sets of native and derivative data have been collected to 2.7 A using a laboratory source.
来自埃及嗜血杆菌的一种DNA（胞嘧啶）-5-甲基转移酶（M.Hae III），可催化从S-腺苷-L-甲硫氨酸到DNA的甲基转移，已与一种自杀性寡核苷酸底物形成共价复合物结晶。共复合物晶体通过悬滴气相扩散法生长，使用聚乙二醇3500作为沉淀剂。晶体属于正交晶系空间群P2(1)2(1)2(1)；晶胞参数为a = 57.6 Å，b = 108.0 Å，c = 155.8 Å，不对称单元中有两个蛋白质-DNA复合物。使用实验室光源已收集到分辨率为2.7 Å的完整天然和衍生数据集。
