限制性内切酶BamHI-DNA复合物的结晶及初步X射线分析

Crystallization and preliminary X-ray analysis of restriction endonuclease BamHI-DNA complex.

作者信息

Strzelecka T, Newman M, Dorner L F, Knott R, Schildkraut I, Aggarwal A K

机构信息

Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032.

出版信息

J Mol Biol. 1994 Jun 10;239(3):430-2. doi: 10.1006/jmbi.1994.1383.

DOI:10.1006/jmbi.1994.1383

PMID:8201623

Abstract

Restriction endonuclease BamHI from Bacillus amyloliquefaciens has been co-crystallized with a 12 bp DNA fragment that encompasses its recognition site. The co-crystals diffract to at least 1.95 A resolution and belong to space group P2(1)2(1)2(1). The unit cell parameters are a = 108.8 A, b = 81.9 A, c = 68.8 A, consistent with one complex in the crystallographic asymmetric unit. The direction of the DNA appears to be along the b axis. In order to achieve end to end stacking of DNA, the complex must lie on the screw axis along b. A self-rotation function has determined the directions of the non-crystallographic 2-fold axes.

摘要

来自解淀粉芽孢杆菌的限制性内切酶BamHI已与一个包含其识别位点的12碱基对DNA片段共结晶。这些共晶体的衍射分辨率至少为1.95埃，属于空间群P2(1)2(1)2(1)。晶胞参数为a = 108.8埃，b = 81.9埃，c = 68.8埃，这与晶体学不对称单元中的一个复合物一致。DNA的方向似乎沿着b轴。为了实现DNA的端到端堆积，复合物必须位于沿着b的螺旋轴上。一个自旋转函数确定了非晶体学2重轴的方向。