Polyribosome profiles and ribonuclease activity in spleens of normal and anaemic mice.

The extent to which the polyribosome content in splenic extracts is affected by the level of endogenous ribonuclease activity was investigated. Ribonuclease activity, free and inhibited, was determined under ionic conditions optimal for preservation of polyribosomes. The results show that ribonuclease activity in control spleens varies mainly with the amount of ribonuclease inhibitor, indicating that free ribonuclease activity is stable. In rapidly proliferating splenic tissue the enzymatic activity is regulated by changes in both the amount of inhibitor and enzyme available. The ribonuclease versus ribonuclease-inhibitor balance was found to parallel the proportion of polyribosomes in a polysome profile.