Roessler B J, Davidson B L
University of Michigan Medical Center, Department of Internal Medicine, Ann Arbor 48109-0680.
Neurosci Lett. 1994 Feb 14;167(1-2):5-10. doi: 10.1016/0304-3940(94)91015-4.
Gene transfer to the central nervous system (CNS) is complicated by the anatomic and physiologic isolation of the brain. Direct injection techniques circumvent this, and allow delivery of transgenes to specific areas of the CNS. Previously, direct transfection of cellular components of the CNS has been achieved using plasmid DNA. We report the use of cationic liposomes as a means of transfecting plasmids into adult mammalian brain. Using the gene for E. coli beta-galactosidase or the cDNA or human beta-glucuronidase as reporters, we demonstrate plasmid mediated gene transfer into the caudate putamen of adult mice with expression of the transgene for at least 21 days post-transfection.
基因转移至中枢神经系统（CNS）因大脑的解剖学和生理学隔离而变得复杂。直接注射技术可规避这一问题，并允许将转基因传递至中枢神经系统的特定区域。此前，使用质粒DNA已实现对中枢神经系统细胞成分的直接转染。我们报告了使用阳离子脂质体作为将质粒转染至成年哺乳动物大脑的一种方法。以大肠杆菌β-半乳糖苷酶基因或cDNA以及人β-葡萄糖醛酸酶作为报告基因，我们证明了质粒介导的基因转移至成年小鼠的尾状壳核，且转基因在转染后至少21天表达。
