Mantovani R, Tora L, Moncollin V, Egly J M, Benoist C, Mathis D
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire de Génie Génétique de l'INSERM, Faculté de Médecine, Strasbourg, France.
Nucleic Acids Res. 1993 Oct 25;21(21):4873-8. doi: 10.1093/nar/21.21.4873.
We have analysed the function of sequences in the TATA/initiator region of the promoter of Ea, a class II gene of the Major Histocompatibility Complex. We find that the Ea promoter contains an initiator element with a strong influence on transcription. We also find that the Ea promoter does contain a bona fide TATA box, which can be recognized by the TATA binding protein (TBP), and that TBP is required for transcriptional activity. For activity, TBP must be included within a larger TFIID complex, as Ea transcription in a heat-treated extract can be restored by immunopurified TFIID but not by TBP alone. On the other hand, the TATA motif can be eliminated without significantly affecting either the efficiency or the startsites of transcription. This suggests that TBP, even in this TATA-containing promoter, is held in place by other components of the initiation complex, regardless of its affinity for the underlying DNA.
我们分析了主要组织相容性复合体II类基因Ea启动子的TATA/起始子区域中序列的功能。我们发现Ea启动子含有一个对转录有强烈影响的起始子元件。我们还发现Ea启动子确实包含一个真正的TATA框，它可以被TATA结合蛋白（TBP）识别，并且TBP是转录活性所必需的。为了实现活性，TBP必须包含在一个更大的TFIID复合物中，因为热处理提取物中的Ea转录可以通过免疫纯化的TFIID恢复，但不能仅通过TBP恢复。另一方面，TATA基序可以被消除而不会显著影响转录效率或起始位点。这表明，即使在这个含有TATA的启动子中，TBP也是由起始复合物的其他成分固定在位的，而不管其对潜在DNA的亲和力如何。
