嗜热栖热菌的依赖DNA的RNA聚合酶；该酶的特性及大亚基基因的DNA序列

The DNA-dependent RNA-polymerase of Thermotoga maritima; characterisation of the enzyme and the DNA-sequence of the genes for the large subunits.

作者信息

Palm P, Schleper C, Arnold-Ammer I, Holz I, Meier T, Lottspeich F, Zillig W

机构信息

Max Planck Institut für Biochemie, Martinsried, Germany.

出版信息

Nucleic Acids Res. 1993 Oct 25;21(21):4904-8. doi: 10.1093/nar/21.21.4904.

DOI:10.1093/nar/21.21.4904

PMID:8177738

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC311404/

Abstract

An improved purification procedure for Thermotoga maritima RNA-polymerase holoenzyme was developed. The enzyme is highly active with poly dAT or T7 phage DNA as template. DNA gyrase was found to be a side product of this RNA-polymerase purification. The genes for the large subunits beta and beta' of RNA-polymerase were cloned and sequenced. The phylogenetic position of T.maritima within the bacterial domain was determined by various methods. It is the lowest bacterial offspring but slightly higher than the chloroplasts.

摘要

开发了一种改进的嗜热栖热菌RNA聚合酶全酶纯化程序。该酶以聚dAT或T7噬菌体DNA为模板时具有高活性。发现DNA促旋酶是这种RNA聚合酶纯化的副产物。克隆并测序了RNA聚合酶大亚基β和β'的基因。通过各种方法确定了嗜热栖热菌在细菌域内的系统发育位置。它是最低等的细菌后代，但略高于叶绿体。