Lois C, Alvarez-Buylla A
Rockefeller University, New York, NY 10021.
Science. 1994 May 20;264(5162):1145-8. doi: 10.1126/science.8178174.
During the development of the mammalian brain, neuronal precursors migrate to their final destination from their site of birth in the ventricular and subventricular zones (VZ and SVZ, respectively). SVZ cells in the walls of the lateral ventricle continue to proliferate in the brain of adult mice and can generate neurons in vitro, but their fate in vivo is unknown. Here SVZ cells from adult mice that carry a neuronal-specific transgene were grafted into the brain of adult recipients. In addition, the fate of endogenous SVZ cells was examined by microinjection of tritiated thymidine or a vital dye that labeled a discrete population of SVZ cells. Grafted and endogenous SVZ cells in the lateral ventricle of adult mice migrate long distances and differentiate into neurons in the olfactory bulb.
在哺乳动物大脑发育过程中，神经元前体细胞从其在脑室区和脑室下区（分别为VZ和SVZ）的诞生地迁移至最终目的地。成年小鼠脑内，侧脑室壁中的SVZ细胞持续增殖，且在体外能够生成神经元，但其在体内的命运尚不清楚。在此，将携带神经元特异性转基因的成年小鼠的SVZ细胞移植到成年受体的大脑中。此外，通过微量注射氚标记的胸腺嘧啶核苷或一种能标记离散SVZ细胞群体的活性染料来检测内源性SVZ细胞的命运。成年小鼠侧脑室中的移植和内源性SVZ细胞会远距离迁移并分化为嗅球中的神经元。
