Lim D A, Fishell G J, Alvarez-Buylla A
The Rockefeller University, New York, NY 10021, USA.
Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14832-6. doi: 10.1073/pnas.94.26.14832.
The mammalian subventricular zone (SVZ) of the lateral wall of the forebrain ventricle retains a population of proliferating neuronal precursors throughout life. Neuronal precursors born in the postnatal and adult SVZ migrate to the olfactory bulb where they differentiate into interneurons. Here we tested the potential of mouse postnatal SVZ precursors in the environment of the embryonic brain: (i) a ubiquitous genetic marker, (ii) a neuron-specific transgene, and (iii) a lipophilic-dye were used to follow the fate of postnatal day 5-10 SVZ cells grafted into embryonic mouse brain ventricles at day 15 of gestation. Graft-derived cells were found at multiple levels of the neuraxis, including septum, thalamus, hypothalamus, and in large numbers in the midbrain inferior colliculus. We observed no integration into the cortex. Neuronal differentiation of graft derived cells was demonstrated by double-staining with neuron-specific beta-tubulin antibodies, expression of the neuron-specific transgene, and the dendritic arbors revealed by the lipophilic dye. We conclude that postnatal SVZ cells can migrate through and differentiate into neurons within multiple embryonic brain regions other than the olfactory bulb.
前脑脑室侧壁的哺乳动物脑室下区（SVZ）在整个生命过程中都保留着一群增殖的神经元前体细胞。出生后及成年SVZ产生的神经元前体细胞迁移至嗅球，在那里它们分化为中间神经元。在此，我们在胚胎脑环境中测试了小鼠出生后SVZ前体细胞的潜能：（i）使用一种广泛存在的遗传标记物，（ii）一种神经元特异性转基因，以及（iii）一种亲脂性染料来追踪出生后第5 - 10天的SVZ细胞在妊娠第15天被移植到胚胎小鼠脑室后的命运。在神经轴的多个水平发现了移植来源的细胞，包括隔区、丘脑、下丘脑，并且在中脑下丘大量存在。我们未观察到其整合入皮质。通过用神经元特异性β - 微管蛋白抗体进行双重染色、神经元特异性转基因的表达以及亲脂性染料显示的树突分支，证实了移植来源细胞的神经元分化。我们得出结论，出生后SVZ细胞能够迁移通过多个胚胎脑区，并分化为除嗅球以外的其他脑区的神经元。
