Frischmuth T, Stanley J
Department of Virus Research, John Innes Institute, John Innes Centre for Plant Science Research, Norwich, United Kingdom.
Virology. 1994 May 1;200(2):826-30. doi: 10.1006/viro.1994.1251.
Beet curly top virus (BCTV) infection is associated with the de novo synthesis of a heterogeneous population of subgenomic viral DNAs. Nicotiana benthamiana plants transformed with a partial repeat of one such subgenomic DNA remain susceptible to infection but produce ameliorated symptoms when agroinoculated with BCTV. Transgenic plants contained from 10 to 30% of the amount of viral DNA detected in nontransformed control plants showing severe symptoms. Symptom amelioration is associated with the mobilization of subgenomic DNA from the integrated template and its amplification to approximately one third of the total amount of viral DNA. The amplification in transgenic plants of a specific subgenomic DNA rather than a heterogeneous population implies that mobilization from the integrated template frequently occurs during systemic infection, precluding the accumulation of other subgenomic DNA forms.
甜菜曲顶病毒（BCTV）感染与亚基因组病毒DNA异质群体的从头合成有关。用一种此类亚基因组DNA的部分重复序列转化的本氏烟草植株仍易受感染，但在接种BCTV时会产生减轻的症状。转基因植物中检测到的病毒DNA量为表现出严重症状的未转化对照植物中病毒DNA量的10%至30%。症状减轻与亚基因组DNA从整合模板的动员及其扩增至病毒DNA总量的约三分之一有关。特定亚基因组DNA而非异质群体在转基因植物中的扩增意味着从整合模板的动员在系统感染期间频繁发生，从而排除了其他亚基因组DNA形式的积累。
