脊髓灰质炎病毒与其细胞表面结合位点的相互作用。

Interaction of poliovirus with its cell surface binding site.

作者信息

Bibb J A, Witherell G, Bernhardt G, Wimmer E

机构信息

Department of Microbiology, SUNY at Stony Brook 11794-8621.

出版信息

Virology. 1994 May 15;201(1):107-15. doi: 10.1006/viro.1994.1270.

DOI:10.1006/viro.1994.1270

PMID:8178474

Abstract

The interaction of poliovirus with its cellular binding sites was characterized by using a receptor-excess silicon oil partition assay. Poliovirus type 1 Mahoney [PV1(M)] binding to HeLa cells fits a theoretical simple bimolecular noncooperative binding curve with an equilibrium dissociation constant (Kd) of 4.3 x 10(7) cells.ml-1 at 4 degrees, or 2.1 x 10(-10) M, assuming 3000 virus binding sites/cell. The association rate of complex formation was measured to be 3.6 x 10(-9) ml.cell-1.min-1 (7.2 x 10(8) M-1.min-1) and the dissociation rate calculated to be 1.5 x 10(-1) min-1, giving the complex a half-life of 4.5 min. The equilibrium dissociation constant, association rate, and dissociation rate were also measured for the binding of the attenuated poliovirus type 3 Sabin strain [PV3(S)] to HeLa cells. PV3(S) bound HeLa cells with a Kd of 3.3 x 10(7) cells.ml-1 (1.6 x 10(-10) M), an association rate of 4.1 x 10(-9) ml.cell-1.min-1 (8.2 x 10(8) M-1.min-1), and a dissociation rate calculated to be 1.4 x 10(-1) min-1, giving the complex a half-life of 5.1 min. Thus the virulent and avirulent strains of poliovirus bind HeLa cells with nearly identical binding constants and rate constants. Equilibrium binding constants for PV1(M) to various other cell types varied from a high affinity of 4.1 x 10(6) cells.ml-1 for JA-1 cells to a low affinity of 7 x 10(7) cells.ml-1 for NGP cells.(ABSTRACT TRUNCATED AT 250 WORDS)

摘要

通过使用受体过量硅油分配测定法对脊髓灰质炎病毒与其细胞结合位点之间的相互作用进行了表征。1型脊髓灰质炎病毒马奥尼株[PV1(M)]与HeLa细胞的结合符合理论上简单的双分子非协同结合曲线，在4℃时平衡解离常数（Kd）为4.3×10⁷个细胞·ml⁻¹，或2.1×10⁻¹⁰M，假设每个细胞有3000个病毒结合位点。复合物形成的缔合速率经测定为3.6×10⁻⁹ml·细胞⁻¹·min⁻¹（7.2×10⁸M⁻¹·min⁻¹），解离速率经计算为1.5×10⁻¹min⁻¹，复合物的半衰期为4.5分钟。还测定了减毒3型脊髓灰质炎病毒萨宾株[PV3(S)]与HeLa细胞结合的平衡解离常数、缔合速率和解离速率。PV3(S)与HeLa细胞结合的Kd为3.3×10⁷个细胞·ml⁻¹（1.6×10⁻¹⁰M），缔合速率为4.1×10⁻⁹ml·细胞⁻¹·min⁻¹（8.2×10⁸M⁻¹·min⁻¹），解离速率经计算为1.4×10⁻¹min⁻¹，复合物的半衰期为5.1分钟。因此，脊髓灰质炎病毒的强毒株和无毒株与HeLa细胞结合的结合常数和速率常数几乎相同。PV1(M)与其他各种细胞类型的平衡结合常数从对JA - 1细胞的高亲和力4.1×10⁶个细胞·ml⁻¹到对NGP细胞的低亲和力7×10⁷个细胞·ml⁻¹不等。（摘要截短于250字）