Inhibition of the antidiuretic effect of 1-deamino-8d-arginine vasopressin (DDAVP) by glibenclamide in water-loaded healthy subjects.

The diuretic effect of a three-day glibenclamide treatment was studied in 8 healthy subjects during maximal sustained water diuresis before and after administration of 0.04 mug l-deamino-8d-arginine vasopression (DDAVP). The antidiuretic response (decrease in free water clearance per 100 ml GFR and increase in urine osmolality) induced by DDAVP was significantly reduced by glibenclamide. In addition, changes in urine osmolality indicated a shorter duration of DDAVP -antidiuresis. These findings are consistent with the hypothesis that glibenclamide competitively inhibits the peripheral action of vasopressin.