[利用早期胚胎多能干细胞构建小鼠嵌合体]
[Formation of mouse chimeras from early embryonic pluripotential stem cell].
作者信息
Song Z, Li Q, Shang K, Wu H
机构信息
Department of Biology, Peking University.
出版信息
Yi Chuan Xue Bao. 1993;20(6):499-503.
PMID:8179936
Abstract
Three chimeric mice were produced by injecting the embryonic stem cell-CCE cells into the cavity of 3.5 day host blastocysts from Kunming and C57BL/6J mice. By analyses of glucose phosphate isomerase (GPI-1), the results show a contribution from CCE cells in heart tissue of female Kunming chimera. Breeding experiments demonstrated that all chimeric mice were fertile but none of 3 chimeras produced germ line transmission.
摘要
通过将胚胎干细胞-CCE细胞注射到来自昆明小鼠和C57BL/6J小鼠的3.5天宿主囊胚腔中，产生了三只嵌合小鼠。通过对葡萄糖磷酸异构酶（GPI-1）的分析，结果显示CCE细胞对雌性昆明嵌合体的心脏组织有贡献。繁殖实验表明，所有嵌合小鼠都具有生育能力，但三只嵌合体中没有一只产生种系传递。
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