Kovacs M S, Lowe L, Kuehn M R
Biologic Resources Laboratory, University of Illinois, College of Medicine, Chicago.
Lab Anim Sci. 1993 Feb;43(1):91-3.
We examined whether superovulation can be used to improve the efficiency of donor embryo collection in embryonic stem cell injection chimera experiments. Superovulation of prepuberal C57BL/6 mice was compared with spontaneous ovulation of mature mice with respect to production and survival of blastocysts. Our results indicate that, compared with spontaneous ovulation, superovulation results in an increased number of blastocysts per female with no difference in viability when transferred to outbred Swiss Webster foster mothers. The advantage of using superovulated females, in terms of maintaining smaller numbers of both females and stud males, is discussed.
我们研究了在胚胎干细胞注射嵌合体实验中，超数排卵是否可用于提高供体胚胎采集的效率。就囊胚的产生和存活而言，我们将青春期前C57BL/6小鼠的超数排卵与成熟小鼠的自然排卵进行了比较。我们的结果表明，与自然排卵相比，超数排卵可使每只雌性小鼠产生的囊胚数量增加，并且在移植到远交系瑞士韦伯斯特代孕母鼠后，其活力没有差异。文中还讨论了使用超数排卵雌性小鼠的优势，即在保持较少数量的雌性和种公鼠的情况下仍能实现上述效果。
