Maione R, Fimia G M, Holman P, Schaffhausen B, Amati P
Dipartimento di Biopatologia Umana, Università di Roma La Sapienza, Italy.
Cell Growth Differ. 1994 Feb;5(2):231-7.
The expression of polyomavirus large T antigen in stably transfected C2 myoblast cells inhibits terminal differentiation without inducing a transformed phenotype. In the present work, we report on the lifting of this inhibition by a mutation that prevents polyomavirus large T antigen from binding to the product of the retinoblastoma susceptibility gene (p105 RB). In contrast with cells containing wild-type large T, those with the Rb binding site mutant large T showed the same up-regulation of myosine heavy chain and myogenin mRNA expression as control cells. Furthermore, we correlate the cell cycle alteration induced by polyomavirus large T antigen expression with the inability of the cells to undergo terminal differentiation.
多瘤病毒大T抗原在稳定转染的C2成肌细胞中的表达可抑制终末分化，而不诱导转化表型。在本研究中，我们报道了一种突变可解除这种抑制，该突变阻止多瘤病毒大T抗原与视网膜母细胞瘤易感基因（p105 RB）的产物结合。与含有野生型大T的细胞相比，具有Rb结合位点突变型大T的细胞与对照细胞一样，肌球蛋白重链和肌细胞生成素mRNA表达上调。此外，我们将多瘤病毒大T抗原表达诱导的细胞周期改变与细胞无法进行终末分化联系起来。
