Fonseca L, de Toledo B E, Orrico S R, Elias A M, Ito I Y
Faculdade de Odontologia, Universidade Federal do Espírito Santo, Vitória, Brazil.
Braz Dent J. 1993;4(1):3-8.
In the present study we investigated the presence of spirochetes in the subgingival plaque of 66 boys and girls aged 5 to 10 years. A total of 264 samples were removed from 4 different sites using wooden wedges held with hemostatic pliers and introduced into tubes containing 0.6 ml reduced transport fluid (RTF). After dispersal of the material, smears were prepared and stained by the Fontana-Tribondeau and Giemsa methods for spirochete visualization. Slides were examined under a binocular light microscope with a 100X objective and at least 200 microorganisms were counted, including spirochetes. Spirochetes were detected in 27.27% of the samples and were absent in 72.73% of them, i.e., 65.15% of the children harbored spirochetes in at least one of the sites studied. The relative frequency of percentage of spirochetes observed was quite low in 6-year old children (9.09%), and was higher in 7- and 10-year old children (41.76% and 36.62%, respectively) than in the remaining age groups studied. The spirochete-age correlation was statistically significant (P < 0.01).
在本研究中，我们调查了66名5至10岁男孩和女孩龈下菌斑中螺旋体的存在情况。使用止血钳夹持的木质楔子从4个不同部位共采集了264份样本，并将其放入含有0.6毫升还原运输液（RTF）的试管中。材料分散后，制备涂片，并用Fontana-Tribondeau法和吉姆萨法染色以观察螺旋体。在双目光学显微镜下用100倍物镜检查载玻片，并计数至少200个微生物，包括螺旋体。在27.27%的样本中检测到螺旋体，72.73%的样本中未检测到螺旋体，即65.15%的儿童在至少一个研究部位携带螺旋体。观察到的螺旋体百分比相对频率在6岁儿童中相当低（9.09%），在7岁和10岁儿童中较高（分别为41.76%和36.62%），高于其他研究年龄组。螺旋体与年龄的相关性具有统计学意义（P < 0.01）。
