Thamavit W, Pairojkul C, Tiwawech D, Shirai T, Ito N
Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Cancer Lett. 1994 Apr 1;78(1-3):121-5. doi: 10.1016/0304-3835(94)90040-x.
Continuous administration of dimethylnitrosamine (DMN) to Syrian hamsters infected with the liver fluke, Opisthorchis viverrini (OV) results in a 100% incidence of cholangiocellular carcinomas. In a two-stage experiment, however, dosing with liver flukes caused only a few lesions to develop (Flavel, D.J. and Lucus, S.B. (1983) Carcinogenesis, 4, 927]. To clarify this anomaly, Syrian hamsters were initiated with 20 mg/kg DMN injected i.p. 19 days prior to 80 OV metacercaria infection. At 45 weeks, the animals receiving both DMN and the parasite demonstrated a 44% incidence of cholangiocarcinomas, a 93% incidence of cholangiofibrosis, a 35% incidence of mucous cystadenomas and a 98% incidence of hepatocellular nodules with an average number of 9.1 +/- 4.1 per animal. Animals receiving DMN alone developed 85% hepatocellular nodules with an average number of only 3.0 +/- 2.7 and no bile duct lesions. In the parasite alone group, only cholangiofibrosis was detected in a few animals and no lesions were encountered in untreated controls. These results thus demonstrate that the post-initiation influence of Opisthorchiasis is indeed effective in promoting the development of both cholangiolar and hepatocellular lesions initiated by DMN.
给感染肝吸虫（华支睾吸虫，OV）的叙利亚仓鼠持续施用二甲基亚硝胺（DMN）会导致胆管细胞癌的发生率达到100%。然而，在一项两阶段实验中，感染肝吸虫仅导致少数病变发生（Flavel, D.J.和Lucus, S.B.（1983年），《癌变》，4，927）。为了阐明这一异常现象，在感染80个OV尾蚴前19天，给叙利亚仓鼠腹腔注射20 mg/kg DMN进行启动处理。在45周时，同时接受DMN和寄生虫感染的动物胆管细胞癌发生率为44%，胆管纤维化发生率为93%，黏液性囊腺瘤发生率为35%，肝细胞结节发生率为98%，每只动物平均有9.1±4.1个。仅接受DMN的动物肝细胞结节发生率为85%，平均数量仅为3.0±2.7，且无胆管病变。在仅感染寄生虫的组中，仅在少数动物中检测到胆管纤维化，未处理的对照组未出现病变。因此，这些结果表明，华支睾吸虫病在启动后的影响确实有效地促进了由DMN引发的胆管和肝细胞病变的发展。
