SacNI, an isoschizomer of BanII isolated from Streptomyces achromogenes recognizes the 5'-GRGCY/C sequence.

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作者信息

Rutkowska S M, Skowron P M, Bielawski K, Podhajska A J

机构信息

Department of Microbiology, University of Gdańsk, Poland.

出版信息

Gene. 1995 May 19;157(1-2):319-20. doi: 10.1016/0378-1119(94)00505-m.

DOI:10.1016/0378-1119(94)00505-m

PMID:7607518

Abstract

SacNI, an isoschizomer of the restriction endonuclease, BanII [Sugisaki et al., Nucleic Acids Res. 10 (1982) 5747-5752], has been isolated from Streptomyces achromogenes N-J-H. SacNI recognizes the palindromic sequence, 5'-GRGCY/C, and cleaves within the recognition sequence, generating a 3' protruding RGCY end (where R = A or G, and Y = C or G).

摘要