来自结晶放线菌的限制性内切酶AvcI的纯化与特性分析
Purification and characterization of the restriction endonuclease AvcI from Actinomyces cristalomycini.
作者信息
Rutkowska S M, Skowron P M, Podhajska A J
机构信息
Department of Microbiology, University of Gdańsk, Poland.
出版信息
Gene. 1995 May 19;157(1-2):317-8. doi: 10.1016/0378-1119(94)00583-e.
PMID:7607517
Abstract
The restriction endonuclease AvcI, an isoschizomer of Sau96I [Sussenbach et al., Nucleic Acids Res. 5 (1978) 1153-1163], was purified from Actinomyces cristalomycini. AvcI recognizes a 5-bp palindromic sequence, 5'-G decreases GNCC and cleaves it after the first G residue producing a 3-nucleotide 5'-overhang.
摘要
限制性内切酶AvcI是Sau96I的同裂酶[Sussenbach等人，《核酸研究》5 (1978) 1153 - 1163]，从结晶放线菌中纯化得到。AvcI识别一个5碱基对的回文序列5'-G↓GNCC，并在第一个G残基后切割，产生一个3核苷酸的5'-突出端。
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