Zhang X H, Chinnappa C C
Department of Biological Sciences, University of Calgary, Canada.
Genome. 1994 Feb;37(1):148-56. doi: 10.1139/g94-019.
A cDNA encoding triose phosphate isomerase (TPI) of Stellaria longipes has been isolated and sequenced. The TPI of S. longipes exhibits high similarity to the TPIs from other species at both the nucleotide and amino acid levels. Southern blot analysis showed that the S. longipes genome contained multiple TPI-like nucleotide sequences and only a low degree of polymorphism was observed among genotypes of different habitats. The levels of TPI mRNA, detected by hybridization to the TPI cDNA, appeared similar between the genotypes. However, different genotypes showed variations in the total TPI activity, reflecting the possible ecological effect on the TPI gene expression. Northern blot hybridization to the cDNA also indicated a higher level of TPI mRNA in leaves than in roots, suggesting tissue-specific expression of TPI gene(s). Phylogenetic analysis of the TPI amino acid sequences from 16 taxa suggested that the TPI from S. longipes was more closely related to cytosolic TPIs from other plants than it was to TPIs from prokaryotes.
已分离并测序了编码长柱繁缕磷酸丙糖异构酶（TPI）的cDNA。长柱繁缕的TPI在核苷酸和氨基酸水平上与其他物种的TPI具有高度相似性。Southern印迹分析表明，长柱繁缕基因组包含多个TPI样核苷酸序列，并且在不同生境的基因型之间仅观察到低度多态性。通过与TPI cDNA杂交检测到的TPI mRNA水平在基因型之间似乎相似。然而，不同基因型在总TPI活性上表现出差异，反映了对TPI基因表达可能的生态影响。与cDNA的Northern印迹杂交也表明，叶片中的TPI mRNA水平高于根中，表明TPI基因存在组织特异性表达。对16个分类群的TPI氨基酸序列进行系统发育分析表明，长柱繁缕的TPI与其他植物的胞质TPI比与原核生物的TPI关系更密切。
