Zhou Y H, Ragan M A
Institute for Marine Biosciences, National Research Council of Canada, Halifax, Nova Scotia, Canada.
Curr Genet. 1995 Sep;28(4):317-23. doi: 10.1007/BF00326429.
cDNAs and an intronless single-copy nuclear gene (TPI1) encoding triosephosphate isomerase have been cloned and sequenced from the marine red alga Gracilaria verrucosa. The predicted amino-acid sequence of TPI1 is readily alignable with those of other known TPIs; 26 of 27 active-site residues and 19 of 26 intersubunit-contact residues are identical between TPIs of G. verrucosa and/or animals and green plants. A partial cDNA sequence of a second TPI gene (TPI2), presumably encoding plastid-localized TPI, was recovered by PCR and demonstrated by phylogenetic analysis to be red algal; no TP12 cDNA or genomic clones could be recovered. Genomic Southern analysis demonstrated that at least two TPI-like genes are present in the nuclear DNA of G. verrucosa.
已从海洋红藻江蓠中克隆并测序了编码磷酸丙糖异构酶的cDNA和一个无内含子的单拷贝核基因（TPI1）。TPI1的预测氨基酸序列很容易与其他已知TPI的序列比对；江蓠与动物和绿色植物的TPI之间，27个活性位点残基中有26个以及26个亚基间接触残基中有19个是相同的。通过PCR获得了第二个TPI基因（TPI2）的部分cDNA序列，推测其编码定位于质体的TPI，系统发育分析表明它是红藻的；未获得TP12 cDNA或基因组克隆。基因组Southern分析表明，江蓠的核DNA中至少存在两个类TPI基因。
