Maestroni G J, Conti A
Center for Experimental Pathology, Istituto Cantonale di Patologia, Locarno, Switzerland.
Int J Immunopharmacol. 1994 Feb;16(2):117-22. doi: 10.1016/0192-0561(94)90067-1.
Adrenergic agents can affect hematopoiesis after syngeneic bone marrow transplantation (BMT) in mice. In particular, chemical sympathectomy by 6-hydroxydopamine and/or administration of various doses of the alpha 1-adrenergic antagonist prazosin were shown to increase the concentration of blood granulocytes, platelets and bone marrow granulocyte--macrophage-colony forming units (GM-CFU), and to induce a granulocytic hyperplasia of the spleen. Here we show that while enhancing myelopoiesis, prazosin decreased the number of spleen B- and T-lymphocytes as well as the number of thymocytes after BMT. The relative proportion of thymocyte subpopulations was not affected, suggesting a non-corticosteroid related mechanism. Furthermore, NK activity and primary cytotoxic T-lymphocyte (CTL) response to Lancy vaxinia virus was impaired in mice treated with prazosin after BMT. When noradrenaline was added in bone marrow cultures, it decreased the number of GM-CFU and such an effect was counteracted by prazosin. These findings suggest that hematopoiesis is under an alpha-adrenergic control.
肾上腺素能药物可影响小鼠同基因骨髓移植（BMT）后的造血作用。具体而言，用6-羟基多巴胺进行化学性交感神经切除术和/或给予不同剂量的α1肾上腺素能拮抗剂哌唑嗪，已显示可增加血液中粒细胞、血小板的浓度以及骨髓粒细胞-巨噬细胞集落形成单位（GM-CFU），并诱导脾脏粒细胞增生。在此我们表明，哌唑嗪在增强骨髓生成的同时，会减少BMT后脾脏B淋巴细胞和T淋巴细胞的数量以及胸腺细胞的数量。胸腺细胞亚群的相对比例未受影响，提示存在一种与皮质类固醇无关的机制。此外，BMT后用哌唑嗪处理的小鼠，其NK活性以及对兰西痘苗病毒的原发性细胞毒性T淋巴细胞（CTL）反应受损。当在骨髓培养物中加入去甲肾上腺素时，它会减少GM-CFU的数量，而这种作用可被哌唑嗪抵消。这些发现表明造血作用受α肾上腺素能控制。
