van Heugten E, Spears J W, Coffey M T, Kegley E B, Qureshi M A
Department of Animal Science, North Carolina State University, Raleigh 27695-7621.
J Anim Sci. 1994 Mar;72(3):658-64. doi: 10.2527/1994.723658x.
To investigate the effect of aflatoxin (AF) and dietary methionine (MET) on immune responses of swine, a total of 288 pigs weaned at 21 d of age were allotted to 12 dietary treatments arranged in a 3 x 4 factorial arrangement in a randomized complete block design. Diets consisted of a corn-soybean meal diet (.95% lysine, .30% MET, and .32% cystine) containing either 0, 140, or 280 ppb of AF and supplemented with either 0, .15, .30, or .45% DL-MET. Immune response measurements were made after the pigs had received their diet for 3 wk. Antibody response to sheep red blood cells (SRBC) was measured 0, 7, and 14 d after i.m. injection of 2.5 mL of a 20% SRBC suspension. Total serum immunoglobulin (Ig) M and IgG were measured using an ELISA. In vivo cellular immunity was measured using a phytohemagglutinin (PHA) skin test. Skin thickness was measured 0, 6, 12, 24, and 36 h after s.c. injection of .1 mL of PHA (1.50 mg/mL). In vitro cellular immunity was measured using a lymphocyte blastogenesis assay. Antibody response to SRBC and serum IgM and IgG concentrations were not affected by dietary treatments. Skin thickness response at 6 h after injection was maximal when .45% MET was added to diets containing 280 ppb of AF, whereas the response was maximal at .30% supplemental MET for the 0 and 140 ppb of AF diets (AF x MET interaction, P < .10). Skin thickness was reduced linearly (P < .10) with increasing dietary AF at 12 and 24 h after PHA injection.(ABSTRACT TRUNCATED AT 250 WORDS)
为研究黄曲霉毒素（AF）和日粮蛋氨酸（MET）对猪免疫反应的影响，将288头21日龄断奶仔猪按随机完全区组设计，分配到12种日粮处理组，采用3×4析因排列。日粮由玉米-豆粕型日粮（赖氨酸0.95%、蛋氨酸0.30%、胱氨酸0.32%）组成，分别添加0、140或280 ppb的AF，并补充0、0.15%、0.30%或0.45%的DL-蛋氨酸。猪采食日粮3周后进行免疫反应测定。肌肉注射2.5 mL 20%绵羊红细胞（SRBC）悬液后0、7和14天测定对SRBC的抗体反应。采用酶联免疫吸附测定法测定血清总免疫球蛋白（Ig）M和IgG。采用植物血凝素（PHA）皮肤试验测定体内细胞免疫。皮下注射0.1 mL PHA（1.50 mg/mL）后0、6、12、24和36小时测量皮肤厚度。采用淋巴细胞增殖试验测定体外细胞免疫。对SRBC的抗体反应以及血清IgM和IgG浓度不受日粮处理的影响。在含280 ppb AF的日粮中添加0.45%蛋氨酸时，注射后6小时的皮肤厚度反应最大，而在AF含量为0和140 ppb的日粮中，补充0.30%蛋氨酸时反应最大（AF×MET交互作用，P<0.10）。PHA注射后12和24小时，随着日粮AF含量增加，皮肤厚度呈线性降低（P<0.10）。（摘要截短至250字）
