Buetow D E
J Protozool. 1976 Feb;23(1):41-7. doi: 10.1111/j.1550-7408.1976.tb05244.x.
The 16S ribosomal RNA of the chloroplast of Euglena gracilis strain Z has been characterized in terms of its 2-dimensional electrophoretic "fingerprint" (T1 ribonuclease). Over 100 spots were resolved on the "fingerprint" and each spot was characterized as to which RNA oligonucleotide fragment(s) is contained. When compared to similar analyses of prokaryotic 16S rRNAs and eukaryotic cytoplasmic 18S rRNAs, the chloroplast 16S rRNA was a typically prokaryotic RNA, but bore little if any relationship to eukaryotic 18S rRNAs. Therefore, the cistrons for chloroplast 16S rRNA are related to the equivalent prokaryotic cistrons, but, apparently, are not related to the equivalent eukaryotic cistrons. Among the organisms available for comparison, the Euglena chloroplast 16S rRNA appears most closely related to the 16S rRNA of the eukaryote, Porphyridium cruentum (a red alga), and at least distantly related to the 16S rRNAs of the blue-green algae and perhaps also to the bacilli.
纤细裸藻Z株叶绿体的16S核糖体RNA已根据其二维电泳“指纹图谱”（T1核糖核酸酶）进行了表征。在“指纹图谱”上分辨出了100多个斑点，并对每个斑点所含的RNA寡核苷酸片段进行了表征。与原核16S rRNA和真核细胞质18S rRNA的类似分析相比，叶绿体16S rRNA是典型的原核RNA，但与真核18S rRNA几乎没有关系。因此，叶绿体16S rRNA的顺反子与相应的原核顺反子相关，但显然与相应的真核顺反子无关。在可供比较的生物中，裸藻叶绿体16S rRNA似乎与真核生物紫球藻（一种红藻）的16S rRNA关系最为密切，与蓝藻的16S rRNA至少有远缘关系，也许还与杆菌的16S rRNA有关系。
