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用限制性内切酶EcoRI分析纤细裸藻叶绿体脱氧核糖核酸。

Analysis of Euglena gracilis chloroplast deoxyribonucleic acid with a restriction endonuclease, EcoRI.

作者信息

Mielenz J R, Milner J J, Hershberger C L

出版信息

J Bacteriol. 1977 May;130(2):860-8. doi: 10.1128/jb.130.2.860-868.1977.

DOI:10.1128/jb.130.2.860-868.1977
PMID:233724
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC235292/
Abstract

Cleavage of chloroplast deoxyribonucleic acid (DNA) of Euglena gracilis Z with restriction endonuclease RI from Escherichia coli (EcoRI) yielded 23 bands upon electrophoresis in gels of agarose. Four of the bands contained twice the stoichiometric amount of DNA. One of these bands contained two similarly sized fragments. The sum of the molecular weight of the 24 different fragments equaled the molecular weight of the circular molecule. The restriction fragments had different buoyant densities, with four having distinctly heavy densities in CsCl. Restriction fragments with a high buoyant density were preferentially lost when broken chloroplast DNA was purified by equilibrium density gradient centrifugation. Hybridization of chloroplast ribosomal ribonucleic acid to intact chloroplast DNA determined that there are two cistrons for 16S and 23S ribosomal ribonucleic acid. These two cistrons are located on six restriction fragments, all of which have buoyant densities greater than the intact molecule of chloroplast DNA.

摘要

用来自大肠杆菌（EcoRI）的限制性内切酶RI切割纤细裸藻（Euglena gracilis Z）的叶绿体脱氧核糖核酸（DNA），在琼脂糖凝胶中进行电泳时产生了23条带。其中四条带所含DNA的化学计量数是其他条带的两倍。这些条带中的一条包含两个大小相似的片段。24个不同片段的分子量总和等于环状分子的分子量。限制性片段具有不同的浮力密度，其中四个在CsCl中具有明显较重的密度。当通过平衡密度梯度离心纯化破碎的叶绿体DNA时，具有高浮力密度的限制性片段优先丢失。叶绿体核糖体核糖核酸与完整叶绿体DNA的杂交确定存在16S和23S核糖体核糖核酸的两个顺反子。这两个顺反子位于六个限制性片段上，所有这些片段的浮力密度都大于叶绿体DNA的完整分子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c9e/235292/2facc5886f2c/jbacter00306-0304-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c9e/235292/15572b6dd259/jbacter00306-0299-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c9e/235292/1c6373a36b87/jbacter00306-0300-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c9e/235292/80a5ed080413/jbacter00306-0302-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c9e/235292/22831365d82f/jbacter00306-0303-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c9e/235292/2facc5886f2c/jbacter00306-0304-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c9e/235292/15572b6dd259/jbacter00306-0299-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c9e/235292/1c6373a36b87/jbacter00306-0300-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c9e/235292/80a5ed080413/jbacter00306-0302-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c9e/235292/22831365d82f/jbacter00306-0303-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c9e/235292/2facc5886f2c/jbacter00306-0304-a.jpg

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引用本文的文献

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Euglena gracilis Chloroplast DNA Codes for Polyadenylated RNA.绿眼虫叶绿体 DNA 编码多聚腺苷酸化 RNA。
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Inhibition of deoxyribonuclease activity in the medium surrounding plant protoplasts.抑制植物原生质体周围培养基中的脱氧核糖核酸酶活性。

本文引用的文献

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Science. 1976 Jul 9;193(4248):158-60. doi: 10.1126/science.193.4248.158.
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Transcriptional origin of Euglena chloroplast tRNAs.眼虫叶绿体tRNA的转录起源
Proc Natl Acad Sci U S A. 1976 Jun;73(6):1984-8. doi: 10.1073/pnas.73.6.1984.
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Plant Physiol. 1978 Aug;62(2):272-5. doi: 10.1104/pp.62.2.272.
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