Calvo W, Alabi R, Nothdurft W, Fliedner T M
Institute of Occupational and Social Medicine, University of Ulm, Germany.
Radiat Res. 1994 May;138(2):260-5.
The immigration of neutrophilic granulocytes into megakaryocytes was studied in the bone marrow of normal and X-irradiated beagles under various exposure conditions. Two groups of dogs received homogeneous total-body irradiation. One group received a dose of 1.6 Gy and the other received a dose of 2.4 Gy (midline tissue). A third group was irradiated from the left side of the body only. This exposure resulted in an inhomogeneous total-body irradiation (entrance dose 3.8 Gy, exit dose 0.9 Gy). A fourth group of animals received partial-body irradiation with a dose of 11.7 Gy delivered to the anterior two-thirds of the body, thereby subjecting about 70% of the hemopoietic marrow to irradiation. Dogs of a fifth group remained unexposed to irradiation and served as controls. The marrow was analyzed in sections of the ribs approximately 1 year after irradiation. The total number of megakaryocytes in one section was evaluated. The number of megakaryocytes showing granulocytes in their cytoplasm was determined and expressed as a percentage. This phenomenon can be explained as cytotoxic immigration of granulocytes into megakaryocytes. It was observed in approximately 1-2% of the megakaryocytes in the marrow of normal dogs. One year after irradiation the value increased to 10-26%. It was observed that neutrophilic granulocytes penetrated only into the large mature megakaryocytes in which the nuclei were mostly pyknotic. This phenomenon may be considered as a late effect of irradiation.
在不同照射条件下，研究了正常和经X射线照射的比格犬骨髓中嗜中性粒细胞向巨核细胞的迁移情况。两组犬接受全身均匀照射。一组接受1.6 Gy的剂量，另一组接受2.4 Gy的剂量（中线组织）。第三组仅从身体左侧进行照射。这种照射导致全身不均匀照射（入射剂量3.8 Gy，出射剂量0.9 Gy）。第四组动物接受局部身体照射，剂量为11.7 Gy，照射身体前三分之二部位，从而使约70%的造血骨髓受到照射。第五组犬未接受照射，作为对照。在照射后约1年对肋骨切片的骨髓进行分析。评估一个切片中巨核细胞的总数。确定细胞质中含有粒细胞的巨核细胞数量，并以百分比表示。这种现象可解释为粒细胞向巨核细胞的细胞毒性迁移。在正常犬骨髓中约1 - 2%的巨核细胞中观察到这种现象。照射1年后，该值增加到10 - 26%。观察到嗜中性粒细胞仅侵入细胞核大多固缩的大型成熟巨核细胞。这种现象可被视为照射的晚期效应。
