Takahashi H, Miyoshi T, Boki K
Department of Public Health, School of Medicine, University of Tokushima, Japan.
Tokushima J Exp Med. 1993 Dec;40(3-4):169-75.
For clinical use of gelatin as a dressing, we determined the water-absorbing capacity of gelatin for isotonic sodium chloride solution (ISCS) and bovine serum albumin-isotonic sodium chloride solution (BSA-ISCS). There was no significant difference between water absorption of gelatin with ISCS and with BSA-ISCS. The water-absorbing capacity of gelatin increased with increasing viscosity: gelatins No. 1 > No. 2 > No. 3. Enormous spaces of 20 to 30 microns or approximately 10 microns in size were observed on the gelatin surface. Scaly materials of 10 to 20 microns in size were also observed. The pores of 10 A in radius were predominant in all the gelatins studied. Since the sizes of cellular blood components range between 2 and 17 microns, they can enter the spaces but not the pores of 10 A in radius. On the other hand, water molecules, the major component of plasma, can enter the pores. Of theoretical sorption isotherms, Henderson's and Chung-Pfost's equations were fitted within the range of relative humidity between 1.5 and 75%. Gelatin No. 1 had the smallest constants n, K, B, and C followed by gelatins No. 2 and No. 3, suggesting that gelatin No. 1 is the most flexible for water and thus is fitted most easily to a raw area such as a wound or burn surface. The study of hemostatic effects of gelatin in rats demonstrated that when used at concentrations more than 50% of the bleeding amount, gelatin absorbed water from blood, and was stuck to and covered the wound surface.
为了将明胶用作敷料进行临床应用，我们测定了明胶对等渗氯化钠溶液（ISCS）和牛血清白蛋白 - 等渗氯化钠溶液（BSA - ISCS）的吸水能力。明胶对ISCS和BSA - ISCS的吸水率之间没有显著差异。明胶的吸水能力随粘度增加而增强：1号明胶>2号明胶>3号明胶。在明胶表面观察到大小为20至30微米或约10微米的巨大空隙。还观察到大小为10至20微米的鳞片状物质。在所研究的所有明胶中，半径为10埃的孔隙占主导地位。由于血细胞成分的大小在2至17微米之间，它们可以进入空隙但不能进入半径为10埃的孔隙。另一方面，血浆的主要成分水分子可以进入孔隙。在理论吸附等温线方面，亨德森方程和钟 - 普福斯特方程在相对湿度1.5%至75%的范围内拟合良好。1号明胶的常数n、K、B和C最小，其次是2号和3号明胶，这表明1号明胶对水的柔韧性最强，因此最容易贴合伤口或烧伤表面等创面。对大鼠进行的明胶止血效果研究表明，当以超过出血量50%的浓度使用时，明胶会从血液中吸水，并粘附并覆盖伤口表面。
