Influence of zinc and copper administration on metal disposition in rats with cadmium-metallothionein-induced nephrotoxicity.

Only a few studies have investigated the effects of the interaction between Cu, Zn, and Cd on the toxicity of Cd in vivo and the metabolism of these metals. The present study is concerned with these effects. Observations of the distribution of these metals in liver and renal cortex and of their excretion in urine were made. Sixty male Wistar rats were divided into 10 groups of six (a full factorial design complemented by a center point and a control group) and were administered Zn (0 or 25 mg/kg body mass), Cu (0 or 12.5 mg/kg), and CdMT (cadmium--metallothionein; 0.1 or 0.4 mg Cd/kg) by sc injection. The rats were given Zn and/or Cu 24 hr prior to the CdMT injection. The data were analyzed by linear multiple regression. After CdMT injection, the Cd retention level was markedly reduced in renal cortex and increased in liver by Cu pretreatment, while the urinary excretion of Cd was significantly lower in these rats. The levels of endogenous Zn in renal cortex and liver increased significantly in rats pretreated with Cu. The production of MT in liver and renal cortex was induced more efficiently by Cu than by Zn. The results obtained may be of importance in understanding the mechanism of CdMT toxicity and the potential influence of Zn and Cu on the chronic nephrotoxicity of cadmium.