Ríman J, Sulová A, Pivec L, Dvorák M
Institute of Molecular Genetics, Academy of Sciences, Prague, Czech Republic.
Acta Virol. 1993 Oct;37(5):320-42.
The early replicative nature of avian myeloblastosis virus core-bound 7 S DNA (AMV DNA), indicated by our preceding findings (Ríman et al., 1993), has been confirmed using various experimental approaches. It has been shown by agarose and polyacrylamide gel electrophoresis that this DNA represents actually a collection of molecules the size of which is strongly reminiscent of the minute early replicative structures found in DNA of sea urchin embryos (Baldari et al., 1978). With such a characteristic correspond, the sequence properties of the individual AMV DNA clones, the majority of which were found to be AT-rich with ARS-like motifs and stretches of A-residues carrying conformational requirements for bending. In comparative hybridization experiments, AMV DNA exhibited the highest homology with chicken leukaemic myeloblast scaffold-bound DNA. Compatible with high replicative activity of AMV DNA was also found its specific [methyl-3H]thymidine radioactivity. The constancy of the virus content of this DNA and its virus age-dependent cleavage changes taking place inside the virus core structure open the question of possible significance of this special host DNA for the reaction machinery represented by the retroviral nucleoprotein core complex.
我们之前的研究结果（里曼等人，1993年）表明禽成髓细胞瘤病毒核心结合7S DNA（AMV DNA）具有早期复制特性，这一特性已通过多种实验方法得到证实。琼脂糖和聚丙烯酰胺凝胶电泳显示，这种DNA实际上是一系列分子的集合，其大小与海胆胚胎DNA中发现的微小早期复制结构极为相似（巴尔达里等人，1978年）。与之对应的是，单个AMV DNA克隆的序列特性，其中大多数富含AT，具有类似ARS的基序以及携带弯曲构象要求的A残基延伸。在比较杂交实验中，AMV DNA与鸡白血病成髓细胞支架结合DNA表现出最高的同源性。还发现AMV DNA的特异性[甲基-3H]胸苷放射性与其高复制活性相符。这种DNA中病毒含量的恒定及其在病毒核心结构内发生的与病毒年龄相关的切割变化，引发了关于这种特殊宿主DNA对于逆转录病毒核蛋白核心复合物所代表的反应机制可能具有何种意义的问题。
