Korb J, Stokrová J, Ríman J, Sulová A
Institute of Molecular Genetics, Academy of Sciences, Prague, Czech Republic.
Acta Virol. 1993 Oct;37(5):343-59.
Structural properties and length distribution profile of 7 S avian myeloblastosis virus (AMV) DNA were studied by means of electron microscopy using two different techniques. This DNA represents mostly double strands, the single strands being in minority. We have shown directly that this DNA forms a bent structure typical of the majority of molecules. These bends are sensitive to the distamycin treatment which stretches most of the bent molecules. Some amount (up to 30%) of circular DNA molecules was detected also in DNA preparations, the nature and the size of which are reminiscent of electron microscopic data on microbubbles of replicating DNA. No specific AMV DNA structural features were found using osmium-tetroxide treatment. The basic size of AMV DNA was estimated to be approximately 150 bp, but its multimers were also detected. Their presence and significance is discussed.
利用两种不同技术，通过电子显微镜研究了7S禽成髓细胞瘤病毒（AMV）DNA的结构特性和长度分布概况。这种DNA大多呈双链，单链占少数。我们直接证明了这种DNA形成了大多数分子特有的弯曲结构。这些弯曲对放线菌素处理敏感，放线菌素处理会使大多数弯曲分子伸展。在DNA制剂中还检测到了一定量（高达30%）的环状DNA分子，其性质和大小让人联想到关于复制DNA微泡的电子显微镜数据。使用四氧化锇处理未发现AMV DNA的特定结构特征。AMV DNA的基本大小估计约为150碱基对，但也检测到了其多聚体。讨论了它们的存在及其意义。
