Nakai K, Matsuda N, Ohta T, Amano M, Takahashi T A, Sakuma I, Kitabatake A, Ito S, Nakazato Y, Sekiguchi S
Hokkaido Red Cross Blood Center, Sapporo, Japan.
Artif Organs. 1994 Mar;18(3):198-205. doi: 10.1111/j.1525-1594.1994.tb02176.x.
Stroma-free hemoglobin (SFH) contains a trace amount of phospholipids, which has been implicated in the toxic reactions associated with SFH. We analyzed stromal phospholipids by high-performance liquid chromatography and found that SFH contained small quantities of lysophosphatidylcholine (LPC), which is known to be capable of producing a defect in endothelium-dependent arterial relaxation, in addition to major classes of constituent phospholipids in red cell membrane. LPC content was determined to be 1.65 nmol/ml (hemoglobin 8.1 g/dl). To evaluate the role of these stromal phospholipids in SFH-induced vasoconstriction, we next examined the effect of lipids on vascular tone in rabbit aortic strips. Preincubation with the crude lipid extract or the LPC purified from SFH significantly inhibited acetylcholine-induced relaxation in phenylephrine-precontracted tissues. The LPC-induced inhibition was reversed by incubation of the tissues in the absence of lipids, indicating the functional integrity of endothelium. From these results, we propose a possibility that LPC, a component of stromal phospholipids, is a candidate for vasoconstrictive factors present in SFH.
无基质血红蛋白（SFH）含有微量磷脂，这与SFH相关的毒性反应有关。我们通过高效液相色谱分析了基质磷脂，发现SFH除了含有红细胞膜中的主要磷脂成分外，还含有少量溶血磷脂酰胆碱（LPC），已知LPC能够导致内皮依赖性动脉舒张功能缺陷。测定LPC含量为1.65 nmol/ml（血红蛋白8.1 g/dl）。为了评估这些基质磷脂在SFH诱导的血管收缩中的作用，我们接下来研究了脂质对兔主动脉条血管张力的影响。用粗脂质提取物或从SFH中纯化的LPC预孵育显著抑制了去氧肾上腺素预收缩组织中乙酰胆碱诱导的舒张。在无脂质条件下孵育组织可逆转LPC诱导的抑制作用，表明内皮功能完整。根据这些结果，我们提出一种可能性，即LPC作为基质磷脂的一种成分，是SFH中存在的血管收缩因子的一个候选因素。
