Kibarer G
Middle East Technical University, Department of Chemistry, Ankara, Turkey.
Artif Organs. 1994 Mar;18(3):222-6. doi: 10.1111/j.1525-1594.1994.tb02180.x.
Spherical activated carbon was employed as a new support material for the immobilization of urease. Activity and kinetic behavior of the enzyme were observed. Urease activity was decreased by 10% for the uncoated samples during the washing process whereas a 30% reduction in activity was observed for the coated samples due to the electrical discharge. Activity versus time plots generally appeared to be s-shaped curves at different initial urea levels. At high substrate concentration the kinetics no longer obeyed the Michaelis-Menten relationship due to the inhibition mechanism and diffusion for the support system.
球形活性炭被用作固定化脲酶的新型载体材料。观察了该酶的活性和动力学行为。在洗涤过程中，未包被样品的脲酶活性降低了10%，而由于放电，包被样品的活性降低了30%。在不同的初始尿素水平下，活性与时间的关系图通常呈S形曲线。在高底物浓度下，由于载体系统的抑制机制和扩散作用，动力学不再遵循米氏关系。
