Pişkin K, Chang T M
Int J Artif Organs. 1980 Nov;3(6):344-6.
A method is described for a new combined enzyme-charcoal system. A model enzyme, urease, was adsorbed on activated charcoal granules which was then coated with ultrathin cellulose nitrate. The assayed activity of this immobilized enzyme was 28.1% /+- 3.12% (mean /+- S.D.) of the activity of urease in solution. The enzyme did not leak out after immobilization and worked efficiently. The assayed urease activity increased with greater amounts of urease/gm immobilized enzyme, but reached a plateau after 40 sumner units/gm. Immobilized urease has good storage and operational stabilities at refrigerator, room and body temperatures. In-vivo studies show that about 90% conversion urea in one single pass is possible.
描述了一种用于新型酶 - 炭组合系统的方法。一种模型酶，即脲酶，被吸附在活性炭颗粒上，然后用超薄硝酸纤维素进行包被。这种固定化酶的测定活性为溶液中脲酶活性的28.1%±3.12%（平均值±标准差）。固定化后酶不会泄漏且能高效发挥作用。测定的脲酶活性随着每克固定化酶中脲酶量的增加而增加，但在达到40萨默单位/克后达到平台期。固定化脲酶在冰箱温度、室温及体温下具有良好的储存稳定性和操作稳定性。体内研究表明单次通过时约90%的尿素转化是可能的。
