Mannella C A, Marko M, Penczek P, Barnard D, Frank J
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
Microsc Res Tech. 1994 Mar 1;27(4):278-83. doi: 10.1002/jemt.1070270403.
The three-dimensional organization of the internal compartments of conventionally fixed and embedded rat-liver mitochondria has been determined by tomographic reconstruction from tilt-series images collected on the Albany high-voltage electron microscope. The results indicate that the inner membranes of these organelles are predominantly tubular in the orthodox (expanded matrix) conformation, as previously suggested by scanning electron microscopy. In the condensed (contracted matrix) conformation, the intracristal space opens up into large irregularly shaped compartments which are connected to each other and to the external (intermembrane) space by tubes with approximately the same diameter (20 nm) as those observed in the orthodox state. These results raise several questions, in particular about the nature of the structural transitions that occur in the cristae during matrix expansion and contraction, and about the influence of inner-membrane shape on the diffusion of ions and metabolites between the intracristal and intermembrane compartments.
通过对在奥尔巴尼高压电子显微镜上收集的倾斜系列图像进行断层重建，确定了常规固定和包埋的大鼠肝脏线粒体内部隔室的三维结构。结果表明，如先前扫描电子显微镜所提示的那样，这些细胞器的内膜在正统（膨胀基质）构象中主要呈管状。在浓缩（收缩基质）构象中，嵴内空间开放形成大的不规则形状的隔室，这些隔室相互连接，并通过与在正统状态下观察到的直径大致相同（20纳米）的管道与外部（膜间）空间相连。这些结果引发了几个问题，特别是关于嵴在基质膨胀和收缩过程中发生的结构转变的性质，以及内膜形状对离子和代谢物在嵴内和膜间隔室之间扩散的影响。
