Gagheş A, Babeş L, Orăşanu M, Codău M L, Teletin N
Cantacuzino Institute, Bucharest, România.
Roum Arch Microbiol Immunol. 1993 Apr-Jun;52(2):89-100.
The structure of the actinic cytoskeleton in Vero cells (African green monkey kidney cells) during monolayer formation was studied. By immunofluorescence after phalloidin staining, actin was visualized in cells 15 min, 2, 4, 24 and 48 hours after cultivation. The evolution in time of cells revealed the progressive organization of actin molecules from diffuse granular forms to filamentous forms with characteristic patterns. The evolution of actinic cytoskeletal elements was closely correlated with the evolution of the cell shape and the extension of intercellular contact surfaces.
研究了单层形成过程中Vero细胞（非洲绿猴肾细胞）中光化性细胞骨架的结构。通过鬼笔环肽染色后的免疫荧光法，在培养后15分钟、2小时、4小时、24小时和48小时的细胞中观察到肌动蛋白。细胞随时间的演变显示肌动蛋白分子从弥漫性颗粒形式逐渐组织成具有特征模式的丝状形式。光化性细胞骨架元件的演变与细胞形状的演变以及细胞间接触表面的扩展密切相关。
