Actin microfilaments dynamics in African green monkey renal cell line (Vero) during cultivation.

The structure of the actinic cytoskeleton in Vero cells (African green monkey kidney cells) during monolayer formation was studied. By immunofluorescence after phalloidin staining, actin was visualized in cells 15 min, 2, 4, 24 and 48 hours after cultivation. The evolution in time of cells revealed the progressive organization of actin molecules from diffuse granular forms to filamentous forms with characteristic patterns. The evolution of actinic cytoskeletal elements was closely correlated with the evolution of the cell shape and the extension of intercellular contact surfaces.