Genari S C, Dolder M A, Wada M L
Department of Cellular Biology, State University of Campinas, UNICAMP, São Paulo, Brazil.
J Submicrosc Cytol Pathol. 1996 Oct;28(4):565-72.
The Vero lineage, established from kidney cells of the green. African monkey, presented fibroblasts-like cells and growth in monolayers. Maintained in culture, the Vero cells presented behavioural and morphologic alterations, associated with cellular transformation. The morphological alterations were investigated using scanning and transmission electron microscopy. The study of proliferation and determination of the cellular doubling time was obtained from the growth curve. The initial population presented growth in a monolayer, while the altered cells grew in multilayers forming cellular aggregates, with flattened cells on the surface and globular cells in the inner region of the aggregate, together with extracellular matrix material. The cell surface of the altered population presented innumerable structures similar to little vesicles, microvilli and cytoplasmic prolongations. The cellular proliferation of both populations was very similar. Our results indicate that morphological and growth changes probably resulted from cellular transformation of the initial Vero cells. These transformed cells presented several characteristics associated with neoplastic growth, and can be used as a model for tumor cells studies in vitro.
Vero细胞系源自非洲绿猴的肾细胞，呈现出成纤维细胞样形态且能单层生长。在培养过程中，Vero细胞出现了与细胞转化相关的行为和形态改变。利用扫描电子显微镜和透射电子显微镜对形态改变进行了研究。通过生长曲线获得增殖研究和细胞倍增时间的测定结果。初始细胞群体呈单层生长，而发生改变的细胞则多层生长形成细胞聚集体，聚集体表面细胞扁平，内部区域细胞呈球状，同时伴有细胞外基质物质。发生改变的细胞群体的细胞表面呈现出无数类似于小囊泡、微绒毛和细胞质突起的结构。两个群体的细胞增殖情况非常相似。我们的结果表明，形态和生长变化可能是初始Vero细胞发生细胞转化的结果。这些转化细胞呈现出与肿瘤生长相关的几个特征，可作为体外肿瘤细胞研究的模型。
