对香豆霉素A1耐药的伯氏疏螺旋体中的gyrB突变
gyrB mutations in coumermycin A1-resistant Borrelia burgdorferi.
作者信息
Samuels D S, Marconi R T, Huang W M, Garon C F
机构信息
Laboratory of Vectors and Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840.
出版信息
J Bacteriol. 1994 May;176(10):3072-5. doi: 10.1128/jb.176.10.3072-3075.1994.
Abstract
We have isolated and characterized mutants of Borrelia burgdorferi that are resistant to the antibiotic coumermycin A1, which targets the B subunit of DNA gyrase. Mutants had either 100- or 300-fold higher resistance to coumermycin A1 than wild-type B. burgdorferi. In each case, a single point mutation in the gyrB gene converted Arg-133 to Gly or Ile. Mutations in the homologous Arg residue of Escherichia coli DNA gyrase are also associated with resistance to coumarin antimicrobial agents.
摘要
我们已经分离并鉴定出了对靶向DNA促旋酶B亚基的抗生素香豆霉素A1具有抗性的伯氏疏螺旋体突变体。与野生型伯氏疏螺旋体相比，突变体对香豆霉素A1的抗性高出100倍或300倍。在每种情况下，gyrB基因中的单个点突变都会将Arg-133转换为Gly或Ile。大肠杆菌DNA促旋酶同源Arg残基的突变也与对香豆素类抗菌剂的抗性有关。
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