de Blaquière G E, Curtis J, Pereira J H, Turk J L
Department of Pathology, Royal College of Surgeons of England, London, U.K.
Int J Lepr Other Mycobact Dis. 1994 Mar;62(1):55-63.
The effectiveness of denatured autologous muscle grafts for nerve repair in an experimental model of leprosy was assessed. Nerve damage resembling that caused by Mycobacterium leprae in humans was induced by the injection of cobalt-irradiated M. leprae into the tibial nerve of guinea pigs. At the time of maximum functional loss, caused by the formation of a granuloma within the nerve, the area of damage was excised and a denatured autologous muscle graft was used to repair the nerve. Assessment of nerve regeneration through the graft was made using clinical, electrophysiological and microscopic morphometric analysis at intervals up to 20 weeks. The results were compared with regeneration after grafting of a normal nerve. Clinically, some motor and sensory recovery occurred in all of the graft recipients in the normal nerve by 8 weeks, and by 11 weeks in the recipients of grafts in the granulomatous nerve. Full sensory recovery occurred in all but one animal by 20 weeks. Motor function recovered to near normal levels at 14 weeks after repair of the normal nerve but, at 20 weeks, there was variation in motor recovery after repair of the granulomatous nerve. Electrophysiology showed increased conduction velocity of the nerve fibers at each time-point. The conduction velocity at 8 weeks after grafting of the normal nerve was similar to that at 12 weeks after grafting of the granulomatous nerve. Morphometry showed an increasing number of myelinated fibers repopulating the distal nerve up to 20 weeks. Myelin fiber numbers, at this time, were one third of normal after repair of the granulomatous nerve and two thirds after repair of the normal nerve.(ABSTRACT TRUNCATED AT 250 WORDS)
评估了变性自体肌肉移植物在麻风病实验模型中对神经修复的有效性。通过将钴辐照的麻风分枝杆菌注射到豚鼠的胫神经中，诱导出类似于人类麻风分枝杆菌引起的神经损伤。在因神经内形成肉芽肿导致功能丧失最大时，切除损伤区域，并用变性自体肌肉移植物修复神经。在长达20周的时间间隔内，通过临床、电生理和显微镜形态计量分析对通过移植物的神经再生进行评估。将结果与正常神经移植后的再生情况进行比较。临床上，正常神经移植物接受者在8周时出现了一些运动和感觉恢复，肉芽肿神经移植物接受者在11周时出现了运动和感觉恢复。到20周时，除一只动物外，所有动物均实现了完全感觉恢复。正常神经修复后14周运动功能恢复到接近正常水平，但在20周时，肉芽肿神经修复后的运动恢复存在差异。电生理学显示在每个时间点神经纤维的传导速度都有所增加。正常神经移植后8周的传导速度与肉芽肿神经移植后12周的传导速度相似。形态计量学显示，在长达20周的时间里，重新填充远端神经的有髓纤维数量不断增加。此时，肉芽肿神经修复后有髓纤维数量为正常的三分之一，正常神经修复后为三分之二。（摘要截断于250字）
